[TP-003]
The Effect of Platelet Rich Plasma on Peyronie's Disease in Rat Model
Mehmet Gökhan Çulha1, Erkan Erkan1, Tuğçe Çay2, Uğur Yücetaş12Department of Pathology, Bagcilar Training & Research Hospital, Istanbul, Turkey
Introduction and AIM: Peyronie’s disease (PD) is a localized connective tissue disorder that involves the tunica albuginea (TA) of the penis. While surgical correction remains the gold standard, the search for an effective and less invasive therapy continues. The objective of this study was to evaluate the effects of intratunical injection of platelet rich plasma (PRP) for the treatment of PD in a rat model.
MATERIAL-METHOD: Twenty male Sprague–Dawley rats (300–350 g) were randomly divided into four groups: sham, PD, PD + PRP and PRP. All rats underwent penile injections into the TA with 0.1 ml vehicle (sham) or 0.5 𝜇g transforming growth factor (TGF)-β1. The PD + PRP groups received intratunical injections with 0.1 ml PRP on day 15 (treatment) or day 0 (PRP effect). Forty-five days following TGF-β1 injection, rats underwent pathological examination. Tissues were evaluated histologically for fibrosis grade (Haematoxylin & Eosin staining), collagen / smooth muscle ratio (Masson Trichrome staining) and type III / type I collagen ration (Picro-sirius red staining). Statistical analysis was performed by Kruskal-Wallis and Chi-Square followed by the Mann-Whitney U test for post hoc comparisons.
RESULTS: Significant changes were found in all three groups compared to the control group (p<0.0001 for fibrosis, p=0.001 for collagen / smooth muscle ratio and p=0.003 for type III / type I collagen ratio). The values in the PRP group and the findings in the PD group are similar (p=0.122 for fibrosis, p=0.221 for collagen / smooth muscle ratio and p=1,0 for type III / type I collagen ratio)
CONCLUSION: PRP has no therapeutic effect on PD. As a result of pathological examinations, PRP shows PD-like effects in rats. PRP may be a cheap, easily accessible and effective disease model for PD treatment research.
Resim-1
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Tunica albugineal injections in midpenil region.