P-087 (Ref.0354): Ratlarda testosteron seviyesi üzerine testiküler kriyoablasyonun etkisi: Histopatolojik orşiektominin deneysel modeli

The Effect of Testicular Cryoablation on Testosterone Level in Rats: An Experimental Model of Histopathological Orchiectomy

Ömer Gökhan Doluoğlu

¹Ankara Training and Research Hospital, Department of Urology, Ankara
²Ankara Training and Research Hospital, Department of Patology, Ankara
³Ankara Training and Research Hospital, Department of Biochemistry, Ankara

PURPOSE: To determine whether testicular cryoablation causes histopathological orchiectomy, and its effect on total testosterone (t-testosterone) levels in rats.
METHODS: A total of 12 Wistar albino male rats were used in this study. The animals were divided into two groups, as cryoablation (9 rats) and control (3 rats) groups. Only scrotal exploration was performed in the control group. Bilateral cryoablation was performed in the cryoablation group. T-testosterone was studied in both groups before scrotal exploration. Bilateral orchiectomy was performed in both groups 10 days after the cryoablation procedure. T-testosterone was measured immediately before orchiectomy. Removed testes were analyzed for presence of necrosis, inflammation, hyperemia, and edema. Mann Whitney U test was used for intergroup comparisons of continuous variables. p<0.05 was regarded as statistically significant.
RESULTS: Baseline t-testosterone levels were 1.31 (0.78-2.45) ng/ml and 0.98 (0.91-2.05) ng/ml in the cryoablation and the control groups, respectively (p=0.92). T-testosterone levels were 0.23 (0.07-1.12) ng/ml and 2.87 (0.63-3.06) ng/ml in the cryoablation and the control groups, respectively, in the blood samples obtained at the time of orchiectomy (10 days after cryoablation) (p=0.03) (Table 1). Histopathological examination of rat testes revealed varying degrees of paratesticular inflammation and necrosis in 13 of 18 testes in the cryoablation group. None of 6 testes showed necrosis in the control group (Table2).
CONCLUSIONS: Our study showed that histopathological orchiectomy could be obtained in the testis by cryoablation. In our opinion, cryoablation can be an alternative to medical and surgical (orchiectomy) castration.

Gruplar arası total testosterone seviyelerinin karşılaştırılması

	Kriyoablasyon grubu (9 rat)	Kontrol grubu (3 rat)	p değeri
Kriyoablasyon öncesi t-testosteron (ng/ml)	1.31 (0.78-2.45)	0.98 (0.91-2.05)	0.92
Orşiektomi sırasında t-testosteron (ng/ml)	0.23 (0.07-1.12)	2.87 (0.63-3.06)	0.03*
Orşiektomiden 72 saat sonra t-testosteron (ng/ml)	0.05 (0.04-0.43)	0.01 (0.01-0.01)	0.38

Comparison of total testosterone (t-testosterone) levels in cryoablation and the control groups.

	Cryoablation group (9 rats)	Control group (3 rats)	p value
t-testosterone before cryoablation (ng/ml)	1.31 (0.78-2.45)	0.98 (0.91-2.05)	0.92
t-testosterone at the time of orchiectomy (ng/ml)	0.23 (0.07-1.12)	2.87 (0.63-3.06)	0.03*
t-testosterone 72 hours after orchiectomy (ng/ml)	0.05 (0.04-0.43)	0.01 (0.01-0.01)	0.38

Histopatolojik bulgular

	Aktif inflamasyon	Aktif inflamasyon	Aktif inflamasyon	Aktif inflamasyon	Hiperemi	Hiperemi	Hiperemi	Hiperemi	Ödem	Ödem	Ödem	Ödem	Paratestiküler inflamasyon ve nekroz	Paratestiküler inflamasyon ve nekroz	Paratestiküler inflamasyon ve nekroz	Paratestiküler inflamasyon ve nekroz
Grup	0	+1	+2	+3	0	+1	+2	+3	0	+1	+2	+3	0	+1	+2	+3
Kriyoablasyon (n=18)	0	4	2	12	0	5	8	5	0	4	13	1	5	1	1	11
Kontrol (n=6)	5	1	0	0	3	3	0	0	3	2	1	0	6	0	0	0
p değeri	0.0001				0.001				0.003				0.004

Histopathological findings

	Active inflammation	Active inflammation	Active inflammation	Active inflammation	Hyperemia	Hyperemia	Hyperemia	Hyperemia	Edema	Edema	Edema	Edema	Paratesticular inflammation and necrosis	Paratesticular inflammation and necrosis	Paratesticular inflammation and necrosis	Paratesticular inflammation and necrosis
Group	0	+1	+2	+3	0	+1	+2	+3	0	+1	+2	+3	0	+1	+2	+3
Cryoablation (n=18)	0	4	2	12	0	5	8	5	0	4	13	1	5	1	1	11
Control (n=6)	5	1	0	0	3	3	0	0	3	2	1	0	6	0	0	0
p value	0.0001				0.001				0.003				0.004

[P-087]

Ratlarda testosteron seviyesi üzerine testiküler kriyoablasyonun etkisi: Histopatolojik orşiektominin deneysel modeli

The Effect of Testicular Cryoablation on Testosterone Level in Rats: An Experimental Model of Histopathological Orchiectomy